模拟微重力条件下大鼠神经干细胞鉴定及培养

he aim of this study is to identify and culture rat neural stem cells under simulated microgravity. Neural stem cells from hippocampus of neonatal SD rats were cultured under clinostat-simulated microgravity and then identified by immunocytochemistry. The morphology of neural stem cells cultured for 1-5 days under simulated microgravity was also detected. Compared with control groups, the results showed that neural stem cells cultured under simulated microgravity were Nestin positive by immunocytochemistry, had the potential of forming clones and can be induced to differentiate into neurons and astrocytes. And the neurospheres formed under simulated microgravity are much larger than the normal gravity control gruop. Therefore, the neural stem cells cultured under simulated microgravity, which have the potential of self-renewal and differentiation, are much easier to form large neurospheres than the normal gravity control gruop.