西瓜和甜瓜蔓枯病菌分子检测技术研究

Gummy stem blight, caused by Didymella bryoniae, is one of the most important worldwide soil-borne diseases of watermelon and melon, and to found rapid and efficient methods of disease detection has huge significance for timely prevention and control. Considering that it is a seed-borne and soil-borne disease, the article screened couples of primers that suitable for PCR and real-time PCR, then used them to detect plant tissues of watermelon that inoculated with disease, seeds of watermelon and melon, and soil that inoculated with disease. The results show that PCR and real-time PCR can detect whether plant infected or not before disease occurring, whether the watermelon and melon seeds on the market carrying diseases or not, and whether melon soils containing disease or not, so that providing reference for GSB prevention and control.