L-氨基酸脱氨酶的分类、结构、功能、应用
Classification, structure, function, and application of L-amino acid deaminase
L-氨基酸脱氨酶（LAAD）催化L-氨基酸氧化脱氨基时，还原的辅因子将电子转移到膜结合的受体上，不会产生有危害的过氧化氢，而且该过程可与ATP产生耦合，异源表达的宿主范围比较广，非常适合用于 α-酮酸及其衍生物的工业化制备，具有高效、环保和成本低的特点。每种变形杆菌都表达两种不同类型的膜相关LAAD，其结构主要包括：FAD结合域、底物结合域、假定的N-末端跨膜α-螺旋和"插入模块"。LAAD 还可以参与许多级联反应，与其他酶串联用于L-氨基酸的生物催化转化为各种产物。
生物化学生物工程学微生物学
L-氨基酸脱氨酶，α-酮酸，黄素蛋白
谭之磊,赵宁,侯颖,孙宇婷.L-氨基酸脱氨酶的分类、结构、功能、应用[EB/OL].(2021-05-27)[2025-10-31].http://www.paper.edu.cn/releasepaper/content/202105-213.点此复制
When L-amino acid deaminase (LAAD) catalyzes the oxidative deamination of L-amino acid, the reducing cofactor transfers electrons to the membrane bound receptor, which will not produce harmful hydrogen peroxide. Moreover, this process can be coupled with ATP production, and the heterologous expression of LAAD has a wide range of hosts, which is very suitable for application α- The industrial preparation of ketoacid and its derivatives has the characteristics of high efficiency, environmental protection and low cost. Each Proteus expressed two different types of membrane associated LAAD, which mainly include FAD binding domain, substrate binding domain and putative N-terminal transmembrane domain α- Screw and "insert module". LAAD can also take part in many cascade reactions, which can be used in tandem with other enzymes for the biocatalytic conversion of l-amino acids to various products.
L-amino acid deaminase α-ketoacid flavin
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