中国HIV -1 vpr 基因多态性及其临床意义的研究

Objective To compare the differences of the mutation sites on human immunodeficiency virus type 1 vpr gene via analyzing the sequences of HIV-1 infected individuals from different regions in China, and select some mutation sequences to carry out the next study according to the clinical features of the infected individuals. Methods Reverse transcription polymerase chain reaction (RT-PCR) and nested PCR was used to amplify HIV-1 vpr gene which extracted from plasma of 398 HIV-1 infected individuals. Using BLAST, ClustalW and Mega biological analysis software to analyze sequence, and identify the characteristics of HIV-1 vpr gene polymorphism and its clinical significance in China. Results 153 positive samples which were obtained from 398 HIV-1 infected individuals were available for further analysis. The sequence detection results were showed as follows: CRF01-AE is 51.63%, subtype C 24.84%, subtype B 17.65%, CRF03-AB 3.92%, CRF08-BC 1.31%. The linear analysis demonstrated that the variation in the C-term was more obvious than which in the N-term. We have discovered that the amino acids mutation sites in 55, 63, 70, 85, 86, 89 and 94 are likely related to the clinical manifestation of HIV-1 infected individuals. Conclusions M group is the main group in China, and CRF01-AE is the main subtype which differs from the previous reports. The proportion of the glumatic acid（Q） is 84.3％ in the 77th position of the 153 amino acid sequences, but some overseas researches reported that the 77th site is arginine（R）and the mutation of R77Q is correlated with long-term nonprogressions. The mutations at some sites of HIV-1 Vpr are possibly correlated with clinical manifestation of HIV-1 infected individuals.