牙鲆消化道组织学观察及内分泌细胞分布

he digestive tract histology structure of Paralichthys olivaceus and three types of endocrine cells were studied by using H.E staining and immunocytochemical methods. The results showed that the histological structure was similiar to that of high vertebrate animals. The tract consisted of oesophagus, stomach, pylorus-theca, and intestine. The intestine was divided into fore-intestine, mid-intestine and hind-inrestine although there was no obvious limit between them. Stomach and fore-intestine were the main region of digestive and absorption. Serotonin and P substance immunoreactive endocrine cells were found in stomach, there are 2.2 and 2.0 cells in every 104μm2 epithelium. Gastrin immunoreactive cells were founded in pylorus-theca and fore-intestine, there are 0.98 and 0.71 cells in every 104μm2 epithelium. Three types of cell were mainly located in the epithelium of stomach or intestine. It showed that there were also many peptidergic neurons in the gastrointestinal tract of Paralichthys olivaceus as in the mammalian and which provided morphological basis for the resource of neuro-endocrine system.