The human PDZome 2.0: characterization of a new resource to test for PDZ interactions by Yeast Two-Hybrid
The human PDZome 2.0: characterization of a new resource to test for PDZ interactions by Yeast Two-Hybrid
Abstract PSD95-disc large-zonula occludens (PDZ) domains are globular modules of 80-90 amino acids that co-evolved with multicellularity. They commonly bind to carboxy-terminal sequences of a plethora of membrane-associated proteins and influence their trafficking and signaling. We previously built a PDZ resource (PDZome) allowing to unveil human PDZ interactions by Yeast two-hybrid. Yet, this resource is partial according to the current knowledge on the human PDZ proteome. Here we built the PDZome 2.0 library for Yeast two-hybrid, based in a PDZ library manually curated from online resources. The PDZome2.0 contains 305 individual clones (266 PDZ domains in isolation and 39 tandems), for which all boundaries have been designed based on available PDZ structures. Using as bait the E6 oncoprotein from HPV16, a known promiscuous PDZ interactor, we show that PDZome 2.0 outperforms the previous resource.
Vincentelli Renaud、Lembo Fr¨|d¨|rique、Trave Gilles、Castro-Cruz Monica、Zimmermann Pascale、Borg Jean-Paul
Architecture et Fonction des Macromol¨|cules Biologiques (AFMB), Unit¨| Mixte de Recherche (UMR) 7257, Centre National de la Recherche Scientifique (CNRS) Aix-Marseille Universit¨|¨|quipe Labellis¨|e Ligue 2018, Centre de Recherche en Canc¨|rologie de Marseille (CRCM), Aix-Marseille Universit¨|¨|quipe Labellis¨|e Ligue 2015, Department of Integrated Structural Biology, Institut de G¨|n¨|tique et de Biologie Mol¨|culaire et Cellulaire (IGBMC), INSERM U1258/CNRS UMR 7104/Universit¨| de StrasbourgDepartment of Human Genetics, KU Leuven||¨|quipe Labellis¨|e Ligue 2018, Centre de Recherche en Canc¨|rologie de Marseille (CRCM), Aix-Marseille Universit¨|Department of Human Genetics, KU Leuven||¨|quipe Labellis¨|e Ligue 2018, Centre de Recherche en Canc¨|rologie de Marseille (CRCM), Aix-Marseille Universit¨|Marseille Proteomics Platform, CRCM, Institute Paoli-Calmettes, Aix-Marseille Universit¨|, Inserm, CNRS
生物科学研究方法、生物科学研究技术基础医学分子生物学
PDZPDZomeprotein-protein interactionHPV16-E6Yeast two-hybrid
Vincentelli Renaud,Lembo Fr¨|d¨|rique,Trave Gilles,Castro-Cruz Monica,Zimmermann Pascale,Borg Jean-Paul.The human PDZome 2.0: characterization of a new resource to test for PDZ interactions by Yeast Two-Hybrid[EB/OL].(2025-03-28)[2025-04-27].https://www.biorxiv.org/content/10.1101/2020.08.06.239343.点此复制
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