浙贝精油化学成分和抑菌活性研究
hemical composition and antimicrobial activities of essential oil from F.thunbergii Miq.
目的：鉴定浙贝母精油的化学成分和抑菌活性。方法：水蒸汽蒸馏法制得精油，采用GC/MS仪自动分析结合保留指数分析化学成分；琼脂扩散法鉴定精油的抑菌活性。结果：分离得21种化学成分，可鉴定成分15种，占精油总离子流色谱峰面积的97.48%；对金黄色葡萄球菌和大肠杆菌均不敏感；对白色念珠菌抑菌圈直径为10.9mm。结论：水蒸气蒸馏浙贝精油主要成分为正十六酸（53.46%），(E,E)-9,12-十八烷二烯酸甲酯（26.96%），油酸（9.34%）；对金黄色葡萄球菌和大肠杆菌无抑制效果，对白色念珠菌为中度敏感。
he essential oil from dried bulbs of F. thunbergii Miq. obtained by hydrodistillation was analyzed by GC-MS combining with Kovats retention index. The results show that major constituents of this oil were n-Hexadecanoic acid (53.46%), (E, E)-9, 12-Octadecadienoic acid (26.96%) and Oleic Acid (9.34%) and 21 chemical components in the oil were separated and 15 chemical components were identified which represented 97.48% of the total peak. Our result showed that this oil have a moderate antifungal activity against Canidia albicans and no antimicrobial activity to Escherichia coli and Staphylococcus aureus at the concentration of 1×106 CFU/ml.
竺锡武、谭琳、曹跃芬
药学基础医学生物科学研究方法、生物科学研究技术
浙贝母精油GC/MS抑菌活性
F. thunbergii MiqEssential oilGC/MSAntimicrobial activity
竺锡武,谭琳,曹跃芬.浙贝精油化学成分和抑菌活性研究[EB/OL].(2010-10-22)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/201010-429.点此复制
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