Plk4 triggers autonomous de novo centriole biogenesis and maturation
Nabais Catarina 1Pessoa Delphine 1van Zanten Thomas 2Mayor Satyajit 2de-Carvalho Jorge 1Telley Ivo A. 1Bettencourt-Dias M¨?nica 1Duarte Paulo 1Carneiro Jorge1
作者信息
- 1. Instituto Gulbenkian de Ci¨oncia (IGC)
- 2. National Centre for Biological Sciences (NCBS)
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Abstract
Abstract
Centrioles form centrosomes and cilia. In most proliferating cells, centrioles assemble through canonical duplication, which is spatially, temporally and numerically regulated by the cell cycle and the presence of mature centrioles. However, in certain cell-types, centrioles assemble de novo, yet by poorly understood mechanisms. Here, we established a controlled system to investigate de novo centriole biogenesis, using Drosophila melanogaster egg explants overexpressing Polo-like kinase 4 (Plk4), a trigger for centriole biogenesis. At high Plk4 concentration, centrioles form de novo, mature and duplicate, independently of cell cycle progression and of the presence of other centrioles. We show that Plk4 concentration determines the kinetics of centriole assembly. Moreover, our results suggest Plk4 operates in a switch-like manner to control the onset of de novo centriole formation, and that distinct biochemical kinetics regulate de novo and canonical biogenesis. Finally, we investigated which other factors modulate de novo centriole assembly and reveal that PCM proteins promote biogenesis, likely by locally concentrating critical components.Key words
Centriole/de novo organelle biogenesis/Plk4/molecular switch/concentration threshold引用本文复制引用
Nabais Catarina,Pessoa Delphine,van Zanten Thomas,Mayor Satyajit,de-Carvalho Jorge,Telley Ivo A.,Bettencourt-Dias M¨?nica,Duarte Paulo,Carneiro Jorge.Plk4 triggers autonomous de novo centriole biogenesis and maturation[EB/OL].(2025-03-28)[2026-04-12].https://www.biorxiv.org/content/10.1101/2020.04.29.068650.学科分类
细胞生物学/分子生物学/生物科学研究方法、生物科学研究技术
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