柚皮苷对脂多糖诱导的beagle犬急性肺损伤的保护作用

Objective: To observe the potential protective effects of naringin on the lung tissue in lipopolysaccharides (LPS)-induced acute lung injury (ALI) mice. Methods: Eighteen beagle dogs were randomly divided into normal control, LPS alone, and LPS+naringin groups. Dogs in LPS+naringin group were pretreatment with naringin at dose of 12.4 mg/kg, while in LPS and normal control groups were dealt with placebos. 1 h latter, LPS (4 mg/kg) or saline was instilled intratracheally followed in 8 h of modeling period. Results: Naringin significantly decreased LPS-induced influx of inflammatory cells, the contents of total protein and cytokines production such as TNF-α and IL-8 in BAL fluid, while significantly but only partially inhibited the activities of MPO and iNOS in lung tissue. Moreover, naringin markedly attenuated LPS-induced increases total sputum volume, as well as pulmonary edema. Conclusion：pre-oral administration of naringin reduces inflammatory responses, pulmonary edema, sputum synthesis, and damage of lung tissue in LPS-induced ALI beagle dogs.