重组人p53腺病毒转染K562/A02源性DC诱导免疫效应研究

Objective To investigate the effects and feasibility of wt-p53 gene in cancer immunotherapy, we examined whether K562/A02 Cells-derived dendritic cells pulsed with the purified full-length wt-p53 protein were capable of inducing the specific antitumor responses against K562/A02 cells in vitro. Methods A human Multidrug Resistant Leukemia cell line K562/A02 with high expression of p-glycoprotein was used. Recombinant adenovirus carrying wild-type p53 gene was transfected into K562/A02 derived dendritic cells in vitro. Uptake of p53 protein by dendritic cells was assessed by Western blotting. Induction of p53-specific CTL response was also evaluated by the cytotoxic assay against K562/A02 cells. Results Western blotting analysis showed the accumulation of p53 protein in wt-p53- transduced dendritic cells. T cells obtained from peripheral blood mononuclear cells of healthy volunteers were stimulated with wt-p53 and then applied to the cytotoxicity assay against the target cells-K562/A02. The CTL activity generated by adenoviral wt-p53-transduced dendritic cells was specific for K562/A02 cells. Conclusion Transfection of wild type p53 gene via Ad-p53 is a potential approach to the gene therapy of leukemia. This may offers a novel and proming immunogene therapeutic srtategy to overcome multidrug resistant leukemia in the future.