病毒诱导的基因沉默载体及其在植物基因功能验证中的应用
evelopment of Virus Induced Gene Silencing Vectors and its Applification for Function Analysis of Gene in Plants
病毒诱导的基因沉默(Virus induced gene silencing , VIGS) 是指携带植物功能基因（片段）的重组病毒在侵染植物后, 可诱导植物内源的同源基因发生沉默，根据表型的变化可以鉴定该基因的生物学功能。本文从病毒诱导的基因沉默(virus induced gene silencing , VIGS) 载体的类型、载体构建及常用的标识基因、VIGS载体的接种方法、沉默表型观察和沉默效率检测、VIGS载体的优点和局限性等几个方面对近年来病毒诱导的基因沉默载体的研究进展进行了综述。
Virus-induced gene silencing (VIGS) is a technology that exploits an antiviral defense mechanism as a tool for function analysis of gene in plants. VIGS circumvents the need for plant transformation, is methodologically simple and yields rapid results. The research advances in development of VIGS vectors and its applification in function analysis of gene in plants are reviewed and prospects.
李大伟、李萃
遗传学分子生物学植物学
病毒诱导的基因沉默载体应用进展
Virus-induced gene silencing (VIGS)Vectorpplificationechnical advance
李大伟,李萃.病毒诱导的基因沉默载体及其在植物基因功能验证中的应用[EB/OL].(2010-02-09)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201002-410.点此复制
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