针灸疗法对EAMG大鼠血清中AchR-ab和IFN-γ表达水平的影响

Objective: Observation the influence of acupuncture treatment to the EAMG rats' serum AchR-ab and IFN-γ expression level, analyzes the mechanism of acupuncture treatment to MG. Method: Use AchRα1 129-145 peptide fragments to immunization Lewis female rats, for establish EAMG rat models. Selected 30 EAMG model rats, were randomly divided into acupuncture treatment group, drug control group, model control group, with 10 rats in each group. The unmodelled 10 rats which were purchased at the same period were set to blank control group. The acupuncture treatment group to be "warming yang and nourishing qi" acupuncture treatment, the drug control group to be the the pyridostigmine treatment, rats in model control group and blank control group were just captured by the same methods with other groups. The contents of AchR-ab and IFN-γ were tested by ELISA. Result: Model control group rats serum AchR-ab, IFN-γ expression level, is obviously higher than that of the blank control group, difference has statistical significance. The acupuncture treatment group and drug control group rats serum AchR-ab, IFN-γ expression level, decrease in model control group, the difference is statistically significant. Conclusion: The "warming yang and nourishing qi" acupuncture can obviously improve the ENMG rat myasthenia gravis symptoms, and reduce the EAMG rats' serum AchR-ab and IFN-γ expression level, thus giving full play to the role of MG treatment.