骨髓间充质干细胞在创面愈合中的作用研究

Objective: To investigate the contribution of bone marrow mesenchymal stem cells (MSCs) Skin wound repair through vein injection. Method: Bone marrow-derived MSCs of SD rats were isolated and cultured in vitro routinely and the fifth passage as taken for identification of specific surface antigens by flow cytometry. Rats were randomly divided into two groups , skin resection wounds were produce in the experiment group . MSCs of were implanted from tail vein both experiment and control. Investigate its gather around the wound site and identify contribution of bone marrow–derived cells to skin wound repair in different time during wound healing. through immunofluorescence. Results: Flow cytometry showed that MSC s expressed CD 29, and CD90 but no CD45. At 1 week after the cells transplantation , the implanted cells are found around and in the wound site in the experiment group, which is not founded in the control groups. Conclusion: Autologous bone marrow mesenchymal stem cells can possibly contribute to skin wound healing.