推拿对坐骨神经损伤大鼠p75NTR的影响
Investigation of Tuina Therapy on p75NTR of Sciatic Nerve Injury Model Rats
目的 观察推拿对坐骨神经损伤大鼠行为学、神经生长因子受体p75NTR的影响，探讨推拿治疗坐骨神经损伤的生物学机制。方法 采用神经夹持损伤的方式建立大鼠坐骨神经损伤模型，通过斜板实验、热痛阈实验观察空白组、假手术组、模型组、模型对照组、推拿组大鼠的行为学变化；通过免疫组织化学染色观察各组大鼠脊髓内p75NTR表达情况，进而统计分析各组间的差异。结果 模型组和模型对照组大鼠行为学检测表明坐骨神经损伤后大鼠的运动及感觉功能明显降低，推拿治疗后斜板实验及热痛阈实验评分明显升高，并在治疗20天后与正常组无显著性差异；模型组、模型对照组及推拿组p75NTR免疫组化表达与正常组相比均有显著性差异，其中模型组及模型对照组p75NTR显著升高，推拿组p75NTR显著下降。结论 推拿治疗可以通过降低神经生长因子低亲和力受体p75NTR释放，从而抑制细胞凋亡机制，最终改善坐骨神经损伤大鼠的运动及感觉功能。
Objective Observe the influence of Tuina therapy to the p75NTR of sciatic nerve injury model rats. Probe the biological mechanisms of Tuina therapy in sciatic nerve injury. Method Use the nerve clamping method to make the sciatic nerve injury model. Observe the oblique board test and thermal withdraw latency test in blank control group, sham-operated group, model control group, sham-Tuina group and Tuina group as behavioral evidences. Observe p75NTR protein by using immunohistochemistry. Result The oblique board test scores of the model group and sham-Tuina group are significantly lower than blank control group, while Tuina group score is much higher. The thermal withdraw latency test scores of the model group and sham-Tuina group are significantly higher than blank control group, while Tuina group score is much lower. The immunohistochemistry shows that the amounts of the p75NTR proteins are all significantly different in model control group, sham-Tuina group and Tuina group. p75NTR is lower in Tuina group. Conclusion Tuina therapy treats the sciatic nerve injury by reducing p75NTR. As a result, the apoptosis mechanism of nerve cell is controlled by Tuina therapy, so the feeling function and motor function of the rats are improved.
姚斌彬、纪倩、吴剑聪、梅旭晖、于天源、鲁梦倩
中医学神经病学、精神病学基础医学
推拿坐骨神经损伤p75神经营养因子受体
uina sciatic nerve injury p75NTR
姚斌彬,纪倩,吴剑聪,梅旭晖,于天源,鲁梦倩.推拿对坐骨神经损伤大鼠p75NTR的影响[EB/OL].(2013-03-07)[2025-08-24].http://www.paper.edu.cn/releasepaper/content/201303-298.点此复制
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