针刺人迎穴对自发性高血压大鼠血压及内皮素、一氧化氮的影响
Effects of Acupuncturing Renying (ST9) on Blood Pressure and ET/NO level of Spontaneously hypertensive rat
目的:研究针刺人迎穴对自发性高血压大鼠(SHR)血压的影响，并通过内皮素(ET)、一氧化氮(NO)水平以探讨其内在机制。方法：选16周龄、雄性、体重220-270g的SHR30只，东京威斯特大鼠（WKY）10只作为对照。将SHR随机分为3组，即空白对照组、人迎组、卡托普利组，每组10只。人迎组采用针刺双侧人迎穴，卡托普利组给予灌胃，空白对照组及WKY大鼠予相同抓取。实验时间每周连续5天，每天1次，休息2天，共4周。将首日，1-4周的每周第5天干预前、干预后1h、干预后6h血压数据用日本软隆BP98A小动物无创血压仪监测鼠尾收缩压（SBP），4周后取材测定ET/NO含量。 结果：人迎组SBP与空白组相比较，在第2、4周干预前有显著下降（P<0.05）在2-4周干预后1h显著下降（P<0.05）；卡托普利组SBP与空白比较，在1-4周干预前有显著下降（P<0.05），而干预后1h均显著下降（P<0.05），在2-3周干预后6h显著下降（P<0.05）；卡托普利组与人迎组SBP 4周内均无明显统计学差异（P>0.05）。3组SHR与WKY组比较ET均显著高于WKY组（P<0.05）；3组SHR与WKY组比较NO均显著低于WKY组（P<0.05）；3组SHR相互比较ET/NO水平均无明显统计学差异（P>0.05），但人迎组与卡托普利组均有好转趋势。结论：针刺人迎穴在一定程度上能降低血压，其降压效果与卡托普利相当。人迎组和卡托普利组血浆中NO表达均升高、ET表达均降低，提示针刺人迎穴降压机制可能与升高NO和降低ET含量相关。
Objective: To detect effects of acupuncturing Renying (ST9) on blood pressure, of spontaneously hypertensive rats (SHRs), and explore its underlying mechanism through change of endothelium (ET)and NO levels. Methods: 30 male SHR rats and 10 WKY rats of 16-week-old (220-270 g) were selected. 30 SHR rats were randomized into 3 groups (model group, acupuncture group and captopril group). Rats in Renying group would be acupunctured on bilateral Renying； Rats in captopril group would be administrated with captopril; SHRs in model group and WKY rats would only be handled and restrained with same intense and strength. All intervention would be implemented from Monday to Friday for four weeks. Blood pressure of the first day and each Friday would be monitored 3 times (before intervention, 1 hour post intervention and 6 hours post intervention) with arteria caudilis sphygmomanometer (Softron BP-98A, made in Japan), and blood sample would be collect for measurement of ET/NO level after four weeks' intervention. Results: On the basis of all blood pressure data collected before intervention, SBP of rats in Renying group at the second and fourth week significantly dropped (P<0.05) compared with those of rats in model group, meanwhile, SBP of rats in captopril group during all four weeks significantly dropped (P<0.05) compared with those of rats in model group. Through analysis of blood pressure data collected 1 hour after invention, SBP levels of Renying group from second to fourth week were significantly lower than those of model group, while, SBP levels of captopril group during all four weeks were significantly lower (P<0.05) compared with those of rats in model group. No significant difference was found on blood pressure level of captopril group and Renying group (P>0.05). ET levels of all SHRs in 3 groups were higher than that of WKY rats (P<0.05); favorable tendency of ET/NO level was shown in Renying group and captopril group, however, no significant difference was shown between 3 SHRs groups(P>0.05). Conclusion: Acupuncturing Renying (ST9) could decrease blood pressure level to some extent, and its antihypertensive effect might contributing to increasing NO level and decreasing ET level.
王舒、宋文婷、李礼、罗丁、高莹、付静静、沈燕、樊小农
中医学基础医学内科学
针刺穴，人迎自发性高血压大鼠
acupunctureRenying (ST9)spontaneously hypertensive rat
王舒,宋文婷,李礼,罗丁,高莹,付静静,沈燕,樊小农.针刺人迎穴对自发性高血压大鼠血压及内皮素、一氧化氮的影响[EB/OL].(2014-12-09)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201412-252.点此复制
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