紫云英苷对成骨细胞MC3T3-E1增殖、分化、矿化和OPG/RANK/RANKL的影响

Objective:This study aimed to evaluate effects of Astragalin on the proliferation, differentiation, calcification and OPG/RANK/RANKL axis in mouse osteoblastic MC3T3-E1 cells.?Methods:MTT assay was applied in the evaluation of MC3T3-E1 cells proliferation. Differentiating actions of Astragalin with different concentrations in cells were investigated through the examination of alkaline phosphatase (ALP) activities by colorimetric method. And bone nodule formation was dectcted via Alizarin red S staining.The proteins expression of OPG and RANKL of MC3T3-E1 was assayed was assayed using ELISA kit. Results:The effective concentration of Astragalin on MC3T3-E1 proliferation was 5 -100μM. In additional, the ALP activity of MC3T3-E1 could be increased by quercetin (5, 10 and 20μM). And The Astragalin at the concentration (5, 10 and 20μM) remarkably increased the bone nodule formation of cells.The Astragalin could also obviously decrease (P＜0.05) the expression of the RANKL, and increase the expression of OPG obviously (P＜0.05). Ultimately, the Astragalin upgraded the ratio of OPG/RANKL (P＜0.05).Conclusion:Astragalin could promote the proliferation and differentiation of osteoblasts, as well as enhance the ratio of OPG to RANKL, which indirectly inhibit bone resorption process. The results indicated that Astragalin may be an effectve treatment for osteoporosis that acts, in part, via a decrease in bone resorption and an increase in bone formation.