穿膜多肽Tat修饰提高掺氮氧化钛的细胞摄入效率及可见光杀伤效应
Enhancement of intracellular delivery and PDT effect of nitrogen-doped TiO2 to living cells by Tat peptide conjugation
氧化钛是一种化学性质稳定、催化活性高、生物相容性好且无毒的半导体材料，在光动力治疗方面具有很好的发展潜力。但由于它只能被紫外光激发，且不溶于水，被细胞摄入的速度较慢，限制了其在光动力治疗方面的应用。本文首先用高温煅烧法制备了掺氮氧化钛(TiO2N)，使其吸收由紫外光扩展到可见光，再通过3-膦酰基丙酸修饰使TiO2N的表面富含羧基(-COOH)，以提高其水溶性。在此基础上，将Tat多肽与3-膦酰基丙酸修饰的TiO2N结合，制备出掺氮氧化钛-Tat纳米复合物(TiO2N-Tat)。本文对这一纳米复合物材料的各项基本特性进行了表征，并将其与宫颈癌HeLa细胞一起培育，应用于可见光杀伤癌细胞，取得了很好的可见光杀伤效果。因此，TiO2N- Tat纳米复合物作为一种光敏剂材料具有很高的研究价值和巨大的应用潜力。
itanium dioxide (TiO2) is a semiconductor of high chemical stability, good photoreactivity, excellent biocompatibility and nontoxicity, which has promising potential in photodynamic therapy (PDT). However, TiO2 can only be activated by ultraviolet lights. Besides, it is not soluble in water, thus is delivered into cells slowly. These problems hinder its development in PDT. In this paper, nitrogen-doped TiO2 nanoparticle (TiO2N) was prepared by calcination, whose absorption edge was expanded from ultraviolet to the visible region. To enhance its dispersibility in aqueous solution, TiO2N nanoparticles were suface modified with carboxyl (-COOH) by adding 3-phosphonopropionic acid. Then, the suface modified TiO2N was conjugated with Tat peptide to prepare TiO2N-Tat nanocomposite. The properties of the nanocomposite were characterized. Its photokilling effect for HeLa cells under visible-light illumination were also studied. This novel TiO2N-Tat nanocomposite showed great potential in the application of PDT.
糜岚、解晋、王天珑、陈玛欣、王培南、潘晓波
基础医学生物化学生物物理学
光动力治疗掺氮氧化钛-Tat纳米复合物表面修饰细胞摄入
photodynamic therapyN-doped TiO2-Tat nanocompositesurface modificationcellular uptake
糜岚,解晋,王天珑,陈玛欣,王培南,潘晓波.穿膜多肽Tat修饰提高掺氮氧化钛的细胞摄入效率及可见光杀伤效应[EB/OL].(2014-01-15)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/201401-703.点此复制
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