水牛卵巢腔前卵泡数量统计与超微结构观察

he objective of the present study was to describe the quantity of preantral follicle of buffalo ovary, as well as the ultrastructural characterization of the preantral follicle. The results showed that: the number of primordial follicle was estimated at 37578.81±11757.16 structures which was extreme significant difference to primary and secondary follicles at 11537.94±1410.17 and 1753.40±1725.81structures respectively（P<0.01）. And the total preantral follicles of buffalo ovaries 50870.14±11387.26were significantly lower than that of the bovine ovaries 113483.32±22616.60（P<0.01). The results of ultrastructure observation showed that there were some desmosome junctions and gap junctions between granulose cells and oocytes. The nucleus was central located, and there were small lipid vacuoles distributed evenly in the cytoplasm. Few cortical granules were visible in the region where contain well-developed rough endoplasmic reticulum and free ribosomes.