以柯萨奇B3病毒3C蛋白酶为靶点的体外药物筛选模型的建立及应用

he establishment of drug screening model targeting coxsackievirus B3 3C protease (CVB3-3C) and screening new inhibitors was investigated. Prokaryotic expression system expressed CVB3-3C protease in vitro. CVB3-3C protease was purified by affinity chromatography and ion exchange chromatography. Fluorescence resonance energy transfer (FRET) was used to examine the target protease activity. Finally, a drug screening model was established.and 768 compounds were screened. Two compounds (MDCE-A008 and MDCE-A043) with high inhibition ratio were selected, IC50 are (60.61±6.26)μmol/L and (105.7±14.88)μmol/L, respectively. The established model targeting CVB3-3C provides technical guarantee for screening effective inhibitors.