半夏芩连汤对干燥综合征模型NOD小鼠AMPK / TNF-α炎性途径的影响

Objective: To investigate the molecular mechanisms of exocrine immune inflammatory injury of Sj?gren Syndrome and the intervention effect of Ban Xia Qin Lian decoction. Methods: Randomly divided the 24 female NOD mice into model group, positive drug group, double dose TCM group and Conventional dose TCM group, with 6 female Balb/c mice as normal control group. The mice of control group and model group were gavaged with deionized water(0.1ml / 10g o d), the positive group was intervened with Tripterygium (10mg / kg o d), the mice in TCM group were gavaged with chinese herbal compound Ban Xia Qin Lian decoction (Containing crude drug 120 g/kgod and 60g/kgod). The intervention and treatment time was 12 weeks. Then collect Serum, bilateral parotids and submandibular glands. samples .To detect AMPKα1、TNF-α mRNA transcriptional level by RT-PCR,；To assess AMPKα1 protein expression levels by western Blot and Immunohistochemical method；To observeTNF-α protein expression levels by ELISA method. Results: RT-PCR test results: compared with the normal group, the AMPKα1 mRNA transcription level was significantly reduced in the model group, TNF-α mRNA transcription level was significantly increased, the difference was statistically significant (P < 0.01). Compared with the model group, AMPKα1 mRNA transcription level was significantly elevated in the positive drug group and TCM groups，TNF-αmRNA transcription level was significantly reduced in the positive drug group and TCM groups, the difference was statistically significant (P < 0.01). Western Blot test results: compared with the normal group, the protein expression level of AMPKα1was decreased in the model group (P < 0.05). Compared with model group, the protein expression level of AMPKα1 was increased in positive drug group and TCM groups (P < 0.05). Immunohistochemical detection results: compared with the normal group, the protein expression level of AMPKα1 was decreased in the model group (P < 0.05). Compared with the model group, the protein expression level of AMPKα1 was not significant in the positive drug group and theTCM groups (P > 0.05). The ELISA test results: compared with the normal group, the protein expression level of TNF-αincreased significantly in the model group (P<0.01). Compared with the model group, the protein expression level of TNF- αwas significantly decreased in the positive drug group and the TCM groups (P<0.01).