端粒酶重建对皮肤成纤维细胞生物节律的调控

he entrainment of the circadian signals to external or suprachiasmatic nucleus stimulation in the peripheral clocks is essential for maintaining the normal function of human body. However, aging will disrupt the entrainment of peripheral circadian rhythms, thus leads to some age associated diseases. Up till now, little is known about the modification of the oscillatory rhythms in aged cells. A recent report showed that cell senescence in vascular human smooth muscle cells (HSMCs) altered circadian rhythms by a disregulation of rhythmic genes expression. Furthermore, this alteration could be reversed by telomerase reconstitution. To test whether telomerase reconstitution can restore disrupted circadian rhythm in other types of senescent cells, we used fibroblasts as cell models to profoundly investigate the relationship between cell senescence and circadian rhythm modulation. We found that the response of rhythmic genes expression to serum stimulation was markedly attenuated in senescent fibroblasts, and telomerase reconstituted fibroblasts reset the circadian oscillation of rhythmic genes expression through activation of P38-CREB pathway. These findings suggested that telomerase reconstitution might be a good way to reset entrainment of peripheral circadian rhythms disrupted in senescent tissues.