补阳还五汤对高血压大鼠肾脏KKS信号通路表达影响

Objective: To investigate the effect of Buyang Huanwu decoction on KKS signal pathway in the kidney of Dahl salt sensitive rats in pre-hypertension. Method: Dahl salt sensitive rats 20 were randomly divided into model group and administration group. Salt resistant rats 10 as normal group. Through high salt feeding to raise blood pressure, blood pressure in rats rose to 120-139/80-89mmHg, stop the high salt feeding, the medicine group of Buyang Huanwu decoction treatment. After 5 weeks, the left kidney tissue of rats was removed, and the KKS signal pathway related genes were detected by fluorescence quantitative PCR. Elisa was used to detect the content of NO. Result: Compared with the normal group, model group, Kng gene expression increased significantly (P<0.05), the expression of KLK and B2R were significantly reduced (P<0.05), decreased the content of NO (P<0.05); compared with the model group, the expression of Kng gene in Chinese medicine group decreased significantly (P<0.05), KLK, B2R gene expression increased significantly (P<0.05), the content of NO increased significantly (P<0.05).Conclusion: The protective effect of Buyang Huanwu decoction on the kidney in the pre-hypertension is closely related to the KKS signaling pathway.