蚕蛹中天然性别储存蛋白2的分离纯化和功能的初步探索

1980s, Japanese scientists discovered a class of storage proteins in silkworm, which related with sex regulation, and the storage proteins are divided into such storage protein 1 (SP1) and storage protein 2 (SP2).In this study, we has found a heat-resistant protein named sex-specific storage protein 2 (SSP2) in chrysalis, it could resistance 80 ℃ temperature, and its amino acid homology with SP2 up to 90.65%. According to the heat-resistant characteristic of the SSP2, obtaining high purity and still maintain their biological activity of natural SSP2, by heating, centrifugation, filtration, and some way, and through the 30% ammonium sulfate precipitation, Superdex 200, Resource Q other purification methods. The combination of flow cytometry and MTT assay, it found that SSP2 on BmN cells with anti-apoptotic effects, and SSP2 could inhibite the cell apoptosis induced by chemical factors, so we speculated, SSP2 may could protect for cells, providing a basis for studying the function of storage protein in silkworm.