苯醌诱导小鼠骨髓lin-细胞毒性作用的研究

Objective To explore the cytotoxicity of lin- cells from mouse bone marrow induced by 1,4-benzoquinone (BQ) and the mechanism of apoptosis. Methods Lin- cells were acquired from mouse bone marrow of ICR mouse's femurs and tibias by magnetic activated cell sorting (MACS). The cells with the concentration of 1×106/ml were exposed to BQ with the concentrations of 0μM, 1μM, 5μM and 10μM for 24h. Cell proliferation inhibition was detected by MTT assay. Apoptosis was determined by double stained assay with Annexin-Ⅴand PI. Caspase-3 mRNA level was determined by real-time PCR. Results Compared with the control group, the cell relative growth rate (RGR) decreased in 5μM and 10μM BQ treated group（P<0.05）;the apoptosis rate of lin- cells treated with 1μM BQ increased significantly（P<0.05）;an increased level of caspase-3 was observed in 1μM BQ treated group while the expression of caspase-3 appeared to be suppressed in 5μM and 10μM BQ treated group relative to control group. Conclusion The maximal non-effect concentration of BQ to mouse bone marrow lin- cells was 1μM, significant apoptosis in mouse bone marrow lin- cells was induced by BQ at the maximal non-effect concentration but not at higher concentrations.