细胞质雄性不育萝卜花药发育的基因表达研究

he anthers in ?owers perform important functions in sexual reproduction. Seldom studies have been made to study anther transcriptomes to explore genes controlling anther development. To analyze the characteristics of gene expression during the male sterile anther degeneration, we produced transcriptomes of anthers of radish (Raphanus sativus L.) at three progressive developmental stages with cDNA-AFLP method. The transcriptomes included at least 2556 unique transcripts, which involved in transcription, signal transduction, DNA synthesis, cell growth and others. The differential developmental pro?les of the transcripts signify extensive regulation of gene expression in the anther during development. The transcriptomes were used to dissect the major abnormality of gene expression of the sterile anther. First, we categorized and charted the developmental pro?les of all transcripts present in the anther; the transcripts of the sterile anther represent obviously difference contrast to the fertile anther. Second, we successfully selected genes from hundreds of transcripts encoding proteins for transcription, signal transduction, materials transport, and pollen wall formation.