杨树蛋白酶抑制剂基因在拟南芥中的过量表达及对小菜蛾的抑制作用

Proteinase inhibitors are important for plant natural defense system, and they are also important candidate genes in genetic engineering for plant insect-resistance. By Agrobacterium mediated transformation, we transferred two proteinase inhibitor genes from Populus (PttKTI and PttLPI) into Arabidopsis for constitutive expression. After screening transgenic Arabidopsis, we choose those with highest expression level to feed the diamondback moth. Bioassay results indicated that insects fed on transgenic Arabidopsis had lower survival rate, cocooning rate, and moth rate than those fed on the control plants. This indicated that these two poplar protease genes could enhance the insect resistance of Arabidopsis, so that offerd a new way to breed genetically modified plants for better insect resistance.