利用SYFPEITHY表位肽预测法联合MAPPP蛋白酶体切割-表位生成分析预测H5N1全蛋白质组保守HLA-A*0201 T细胞九肽表位
Prediction of conserved HLA-A*0201 restricted nonamer-epitopes for H5N1 proteome using SYFPEITHY combined with MAPPP algorisms
目的：预测H5N1保守性CTL表位。方法及结果：本研究选取了GenBank公布的不同国家或地区在不同时间分离的7株H5N1禽流感病毒，利用clustalW对其蛋白质组进行多重序列比对，发现其中NP、M1、HA、PA、PB1及PB2蛋白序列及NA 84-469aa部分相对保守；进而，对这些保守序列进行SYFPEITHY表位肽预测和MAPPP蛋白酶体切割-表位生成预测，找到71条H5N1蛋白质组保守HLA-A*0201限制性 T细胞九肽表位。意义：这些预测的表位为有效地寻找H5N1表位提供了有价值的参考信息，并为进一步的T细胞免疫疫苗设计打下基础。
o predict conserved HLA-A*0201 restricted CTL epitopes in proteome of H5N1 virus. The study chose 7 distinct H5N1 stains isolated from patients of different countries or districts and infected in different years, blastered the proteome sequences submitted to Genbank, and found the sequences of NP, M1, HA, PA, PB1, PB2 and 84-469aa of NA are relatively more conserved among the strains. Furthermore, through SYFPEITHY combined with MAPPP algorisms, 71 HLA-A*0201 restricted nonamer CTL epitopes in these conserved proteins or regions were predicted. These nona-peptides provide useful information and material for further searching for H5N1 T cell epitopes and vaccine development.
刘树林、汪业军、黄鹤
基础医学生物科学研究方法、生物科学研究技术
H5N1L表位预测SYFPEITHY多重序列比对MAPPP
H5N1CTL epitope predictionSYPHETHIMultiple sequence blasterMAPPP
刘树林,汪业军,黄鹤.利用SYFPEITHY表位肽预测法联合MAPPP蛋白酶体切割-表位生成分析预测H5N1全蛋白质组保守HLA-A*0201 T细胞九肽表位[EB/OL].(2006-11-16)[2025-06-12].http://www.paper.edu.cn/releasepaper/content/200611-457.点此复制
评论