柑桔木虱体内细菌多样性的DGGE和RFLP分析

In this paper, The microbial diversity of citrus psyllids (Diaphorina citri) which vectors Liberobacter sp was been analyse, Using 16S rDNA-based molecular approaches of denaturing gradient gel electrophoresis（DGGE）and restriction fragment length polymorphism（RFLP）. Bacterial taxa obtained by RFLP including Pseudomonadaceae, Enterobacteriaceae, Xanthomonadaceae, Burkholderiaceae, Rickettsiaceae, Rhizobiaceae. Syncytium endosymbiont and secondary symbiont of Diaphorina citri can be also obtained by RFLP. Bacterial taxa obtained by DGGE include four families of Proteobacteria and two families of Firmicutes. The result by PCR-DGGE analysis also revealed that the bacterial population of Diaphorina citri is affected by the host＇s food and the host＇s geographic location.And the impact of host＇s food is more obvious than that of geographic location. However, The existence of Syncytium endosymbiont in Diaphorina citri is not affected by the host＇s food and the host＇s geographic location. The Syncytium endosymbiont of Diaphorina citri is a dominant microflora and the microbial diversity in Diaphorina citri.