三氧化二砷联合丹参酮胶囊抗肝癌的作用效果与机制研究

Objective To elucidate the effect of Arsenic trioxide (As2O3) combined with tanshinone capsule on liver cancer xenograft in nude mice and to investigate the possible mechanism. Methods Liver cancer xenograft model was established in nude mice. After the 19 days of different drug intervention, tumor growth curve was drawn and tumor weight between groups were comparied; Collected blood was analysised on blood routine and biochemical routine; TdT-mediated dUTP nick end labeling(TUNEL) detected tissue apoptosis of tumors;The expression of apoptosis related proteins were detected by immunohistochemistry and western blot. Results Growth curve and tumor weight data showed that, with the extension of delivery time, such as As2O3 low dose group, As2O3 positive control group, tanshinone capsule group and combination group could show some tumor suppression effect.Compared with As2O3 low-dose group and tanshinone capsule group, the tumor suppression effect of combination group was more obvious(p<0.01). Tanshinone capsule almost caused no obvious change on nude mice blood. However, As2O3 both dose groups could significantly reduce the white blood cells and red blood cells and increase the content of platelet (p < 0.05 or p <0.01). However the combination group could reverse this trend, even could make the detection index return to normal levels. The apoptosis rate of combination group increase significantly than As2O3 low-dose group and tanshinone capsule group(p<0.01). The combination group could cut the expression of antiapoptotic protein XIAP(p<0.01)and promote the expression of apoptosis proteinPARP and Bax(p<0.01). Conclusion Low-dose As2O3 combined tanshinone capsule has the attenuated synergies effect in the resistance to liver cancer. Inducing liver cancer cell apoptosis and reducing the toxicity to blood is one of its mechanisms.