Computational fluid dynamic analysis reveals the underlying physical forces playing a role in 3D multiplex brain organoid cultures
Computational fluid dynamic analysis reveals the underlying physical forces playing a role in 3D multiplex brain organoid cultures
Abstract Organoid cultivation in suspension culture requires agitation at low shear stress to allow for nutrient diffusion, which preserves tissue structure. Multiplex systems for organoid cultivation have been proposed, but whether they meet similar shear stress parameters as the regularly used spinner flask and its correlation with the successful generation of brain organoids, has not been determined. Herein, we used computational fluid dynamics (CFD) analysis to compare two multiplex culture conditions: steering plates on an orbital shaker and the use of a previously described bioreactor. The bioreactor had low speed and high shear stress regions that may affect cell aggregate growth, depending on volume, whereas the CFD parameters of the steering plates were closest to the parameters of the spinning flask. Our protocol improves the initial steps of the standard brain organoid formation, and organoids produced therefrom displayed regionalized brain structures, including retinal pigmented cells. Overall, we conclude that suspension culture on orbital steering plates is a cost-effective practical alternative to previously described platforms for the cultivation of brain organoids for research and multiplex testing. HighlightsImprovements to organoid preparation protocolMultiplex suspension culture protocol successfully generate brain organoidsComputational fluid dynamics (CFD) reveals emerging properties of suspension culturesCFD of steering plates is equivalent to that of spinner flask cultures
Goto-Silva Livia、Tovar-Moll Fernanda、Rehen Stevens K.、Ayad Nadia M. E.、Herzog Iasmin L.、Lamien Bernard、Orlande Helcio R. B.、Costa Souza Annie da、Junqueira Magno、Domont Gilberto B.、Martins Michele、Ribeiro Sidarta、Silva Nilton P.
D?ˉOr Institute for Research and Education (IDOR). Rua Diniz CordeiroD?ˉOr Institute for Research and Education (IDOR). Rua Diniz Cordeiro||Institute of Biomedical Sciences, Federal University of Rio de JaneiroD?ˉOr Institute for Research and Education (IDOR). Rua Diniz Cordeiro||Institute of Biomedical Sciences, Federal University of Rio de JaneiroD?ˉOr Institute for Research and Education (IDOR). Rua Diniz CordeiroDepartment of Mechanical Engineering, Politecnica/COPPE - Federal University of Rio de JaneiroDepartment of Mechanical Engineering, Politecnica/COPPE - Federal University of Rio de JaneiroDepartment of Mechanical Engineering, Politecnica/COPPE - Federal University of Rio de JaneiroBrain Institute, Federal University of Rio Grande do NorteProteomics Unit, Institute of Chemistry, Federal University of Rio de JaneiroProteomics Unit, Institute of Chemistry, Federal University of Rio de JaneiroProteomics Unit, Institute of Chemistry, Federal University of Rio de JaneiroBrain Institute, Federal University of Rio Grande do NorteDepartment of Mechanical Engineering, Politecnica/COPPE - Federal University of Rio de Janeiro
生物科学研究方法、生物科学研究技术生物工程学生物物理学
brain organoidsmultiplex culturestem cell researchcomputational fluid dynamics analysis
Goto-Silva Livia,Tovar-Moll Fernanda,Rehen Stevens K.,Ayad Nadia M. E.,Herzog Iasmin L.,Lamien Bernard,Orlande Helcio R. B.,Costa Souza Annie da,Junqueira Magno,Domont Gilberto B.,Martins Michele,Ribeiro Sidarta,Silva Nilton P..Computational fluid dynamic analysis reveals the underlying physical forces playing a role in 3D multiplex brain organoid cultures[EB/OL].(2025-03-28)[2025-05-18].https://www.biorxiv.org/content/10.1101/369082.点此复制
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