诱变型内皮抑素的联合应用研究
the study of co-inhibitory effects of mutant endostandin combined with antisense oligonucleotides
［目的］：利用药物的协同效应提高诱变型内皮抑素的抗癌活性，研究诱变型内皮抑素(ES)联合反义核酸(antisense oligonucleotides ,ASODN)对膀胱癌的抑制作用。比较联合用药和单独用药的效果。[方法]：人工合成包含Bcl－2起始位点的反义核酸及无义核酸(non-sense oligonucleotides, NSODN), 以质脂体lipofectin作为载体转染UM-UC-3膀胱癌细胞系，体外实验采用集落形成实验，吖啶橙溴化乙锭（AO/EB）荧光染色实验，观察不同用药组对实体肿瘤的抑制作用。[结果]：细胞集落形成法检测结果表明联合用药组的集落生存率为49.78%，ES组和反义核酸组分别为75.25%和76.32%（P<0.05）.吖啶橙溴化乙锭（AO/EB）荧光染色结果表明，联合用药组的凋亡率（63.5％）与反义组（33.0％）和内皮抑素组（44.5％）相比都有显著性差异（P<0.05）。 [结论]：诱变型内皮抑素联合反义核酸半量联合用药可以明显提高各自单独用药对膀胱癌的抑制作用。
Objective] To investgate the co-inhibitory effect of mutant endostatin and antisense oligonucleotides on bladder cancer. Compare the effect of combined and solo medication of bladder cancer. [Method] The artificially synthesized ASODN include the translation initiation site. They were transfected into bladder cancer cell by Lipofectamine. Cell growth was observed by colonogenic assay and AO/EB fluorescein stain . [Result] Colonogenic assay: the colony survival rate of the combined group is 53.76% while the ES group and ASODN group is 75.25% and 76.32% respectively. In AO/EB fluorescein stain the co-inhibitory effect is higher than the solo one（P<0.05）. [conclution] The co-inhibitory effect of semis mutant endostatin and antisense oligonucleotides on bladder cancer is higher than the solo one.
任明华、任统
肿瘤学基础医学药学
Bcl－2，反义核酸，内皮抑素，膀胱癌，
Bcl-2antisense oligonucleotidesendostatinbladder cancer
任明华,任统.诱变型内皮抑素的联合应用研究[EB/OL].(2013-11-19)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201311-316.点此复制
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