SARS-CoV-2 spike-specific memory B cells express markers of durable immunity after non-severe COVID-19 but not after severe disease
SARS-CoV-2 spike-specific memory B cells express markers of durable immunity after non-severe COVID-19 but not after severe disease
ABSTRACT SARS-CoV-2 infection elicits a robust B cell response, resulting in the generation of long-lived plasma cells and memory B cells. Here, we aimed to determine the effect of COVID-19 severity on the memory B cell response and characterize changes in the memory B cell compartment between recovery and five months post-symptom onset. Using high-parameter spectral flow cytometry, we analyzed the phenotype of memory B cells with reactivity against the SARS-CoV-2 spike protein or the spike receptor binding domain (RBD) in recovered individuals who had been hospitalized with non-severe (n=8) or severe (n=5) COVID-19. One month after symptom onset, a substantial proportion of spike-specific IgG+ B cells showed an activated phenotype. In individuals who experienced non-severe disease, spike-specific IgG+ B cells showed increased expression of markers associated with durable B cell memory, including T-bet, FcRL5, and CD11c, which was not observed after severe disease. Five months post-symptom onset, the majority of spike-specific memory B cells had a resting phenotype and the percentage of spike-specific T-bet+ IgG+ memory B cells decreased to baseline levels. Collectively, our results suggest that the memory B cell response elicited during non-severe COVID-19 may be of higher quality than the response after severe disease.
Garza Rolando、Cantwell Angelene M.、Clarke Kathleen、Tragus Robin E.、Bol Sebastiaan、Bunnik Evelien M.、Reyes Raphael A.、Patterson Thomas F.、Catano Gabriel、Gonzales S. Jake
Department of Microbiology, Immunology and Molecular Genetics, Long School of Medicine, The University of Texas Health Science Center at San AntonioDepartment of Microbiology, Immunology and Molecular Genetics, Long School of Medicine, The University of Texas Health Science Center at San AntonioDepartment of Microbiology, Immunology and Molecular Genetics, Long School of Medicine, The University of Texas Health Science Center at San AntonioDepartment of Medicine, Division of Infectious Diseases, The University of Texas Health Science Center at San Antonio, University Health SystemDepartment of Microbiology, Immunology and Molecular Genetics, Long School of Medicine, The University of Texas Health Science Center at San AntonioDepartment of Microbiology, Immunology and Molecular Genetics, Long School of Medicine, The University of Texas Health Science Center at San AntonioDepartment of Microbiology, Immunology and Molecular Genetics, Long School of Medicine, The University of Texas Health Science Center at San AntonioDepartment of Medicine, Division of Infectious Diseases, The University of Texas Health Science Center at San Antonio, University Health SystemDepartment of Medicine, Division of Infectious Diseases, The University of Texas Health Science Center at San Antonio, University Health SystemDepartment of Microbiology, Immunology and Molecular Genetics, Long School of Medicine, The University of Texas Health Science Center at San Antonio
医药卫生理论医学研究方法基础医学
T-betFcRL5adaptive immunityhumoral immune responseIgMIgG
Garza Rolando,Cantwell Angelene M.,Clarke Kathleen,Tragus Robin E.,Bol Sebastiaan,Bunnik Evelien M.,Reyes Raphael A.,Patterson Thomas F.,Catano Gabriel,Gonzales S. Jake.SARS-CoV-2 spike-specific memory B cells express markers of durable immunity after non-severe COVID-19 but not after severe disease[EB/OL].(2025-03-28)[2025-05-07].https://www.biorxiv.org/content/10.1101/2021.09.24.461732.点此复制
评论