三种胶原膜理化性质以及其降解产物的成骨特性的研究

Objective: The aim of the present study was to evaluate the physicochemical properties of three kinds of collagen membranes from different sources, by evaluating surface topography, porosity, mechanical properties, degradability, contact angle and osteogenesis of degradation products. Methods: The surface topography of three kinds of collagen membranes was observed by scanning electron microscope. The porosity or swelling property was measured by PBS solution immersion.The tensile strength and elongation at break of collagen membranes were measured by universal testing machine.The collagen membranes were degraded in PBS solution, artificial saliva and collagenase solution to messure the degradation rate.The contact angle of collagen membrane was measured by contact angle measuring instrument, and its hydrophilicity was compared. The osteogenic characteristics of the degraded products of three kinds of collagen membrane were studied by cell proliferation and differentiation experiments. Results: the rough surface of Jieya dermis and Bio-Gide showed loose arrangement of collagen fibers, but there were no collagen fibers on the surface of Giteri; the porosity and contact angle of Bio-Gide were relatively smaller, and the hydrophilicity was stronger; the mechanical strength of Jieya dermis and Gitri was higher.The degradation rate of Jieya dermis was the lowest. The effect of cell proliferation and differentiation of degradation products of Jieya dermis and Giteri was more obvious. Conclusion: This paper studies the different physicochemical properties of three kinds of collagen membrane, each of which has its own advantages, and the corresponding collagen membrane can be selected according to the corresponding cases in clinic.