胃泌素对胃癌细胞SGC7901 Reg I 基因转录因子的效应

IObjective To study the effects of gastrin on Reg I (regenerating gene I） gene transcription factors (TFs) in gastric cancer cell SGC7901. Materials and methods Reg I gene promoter 1414bp fragment was cloned from gastric cancer cell SGC7901 genomic DNA by nest-PCR and identified by sequencing. The 1414bp fragment and its Hind Ⅲ digestion 800bp and 614bp fragments were Digaoxin-labeled by random primer assay and used as probes. Gastric cancer cell SGC7901 was incubated with gastrin-G17 for 48h at final concentrations of 10-7mol/L and 10-8mol/L, and the nuclear proteins were extracted, respectively. Effects of gastrin on Reg I gene TFs were detected by Southwestern blot using Digaoxin-labeled 1414bp, 800bp and 614bp as probes, respectively. Results 20 major bands were detected with 1414bp probe, after gastrin incubation, the densities of band 9, 12, 13, 14, 15 and 16 were decreased significantly (P＜0.05). Of the 6 changed bands detected with 1414bp probe, three bands including band 9, 12 and 13 were detected with proximal 614bp probe. The densities of the three bands were also decreased after gastrin incubation (P＜0.05). Three bands including band 9, 12 and 14 were detected with distal 800bp probe. However, only the density of band 14 was decreased after gastrin incubation (P＜0.05). Conclusions The results suggest that Reg I gene may be transcriptional regulated by cooperation of many TFs in gastric cancer cell SGC7901. Decreasing binding activities of some TFs may be one of pathways of gastrin up-regulating Reg I gene expression in gastric cancer cell SGC7901.