田七总皂苷对PCV-2诱导RAW264.7细胞氧化应激的调节作用

IThe objective of the current study is to investigate the effects of Pannax notoginseng saponins（PNS）on oxidative stress induced by PCV-2 infection in RAW264.7 cells. Vitamin C (200 μM) was used as controlled drugs, PCV-2 infected RAW264.7 cells were treated with PNS（50 μg/mL, 100 μg/mL, 200 μg/mL）or Vc, to observe NO, ROS, GSH, GSSG, MPO, XOD, iNOS production, cell viability and ratio of GSH to GSSG at 4 h, 8 h, 12 h, 24 h. MTT method was used to measure the cell viability. The level of NO was determined by Griess regent. ROS production was evaluated with DCFH-DA fluorescence probe. GSH and GSSG levels were determined with fluorescence method in OPT. MPO, XOD, iNOS activity were measured by chemiluminescence assay. The results showed that PCV-2 infection significantly increased the production of NO, ROS, and GSSG, and significantly raised activity of MPO, XOD, iNOS. and significantly decreased the level of GSH and ratio of GSH to GSSG in PCV-2 infected cells. Treatment with PNS at 200 μg/mL significantly reduced the secretion of NO at 4 h and 12 h after PCV-2 infection, the levels of ROS, GSSG and the activity of MPO, XOD, iNOS was significantly decreased at 4h, 8h, 12h, 24h. And the ratio of GSH to GSSG was significantly increased in infected RAW264.7 cells. The results showed that PCV-2 infection resulted in cell oxidative stress and PNS acted against PCV-2 infection by scavenging ROS in RAW264.7 cells and improving the enzyme activity, but the detaild molecular mechanism needed furher research.