脂多糖致人前列腺上皮细胞产生及分泌HMGB1的作用

Objective: To investigate the effect of LPS on the production and secretion of endogenous HMGB1 in prostate epithelial cells. Methods: The cultured prostate epithelial cells were stimulated by proinflammatory cytokine (LPS, 50 μg/ml) at 3, 6, 12, 24, or 48 h, and then culture medium was collected at each time point. Western blotting and ELISA were used to assay the endogenous production of HMGB1 in human prostate epithelial cells. Results: At 12h, 24h and 48h after LPS administration, the HMGB1 protein expression of cultured prostate epithelial cells were significantly increased compared with the 0h after LPS administration (P<0.05). At 24h and 48h after LPS administration, the HMGB1 protein expression of cultured prostate epithelial cells were also increased compared with the 3h after LPS administration (P<0.05). However, there was no significant difference in the HMGB1 protein expression of cultured prostate epithelial cells among the 12h, 24h and 48h (P>0.05). At 12h and 24h after LPS administration, the HMGB1 protein concentration of medium in cultured prostate epithelial cells were significantly increased compared with the 0h and 3h after LPS administration (P<0.05). As compared to the 6h after LPS administration, the HMGB1 protein concentration of medium in cultured prostate epithelial cells was increased only at 24h after LPS administration (P<0.05). There was no significant difference in the HMGB1 protein concentration of medium in cultured prostate epithelial cells between at 12h and 24h after LPS administration (P>0.05). Conclusion: It can be concluded that HMGB1 protein expression can be up-regulated by LPS, and HMGB1 protein can be secreted into the extracellular.