一个新的家蚕BmUFP基因的序列分析和原核表达
Sequence analysis and prokaryotic expression of a novel BmUFP gene from silkworm,Bombyx mori
从本实验室构建的家蚕cDNA文库中克隆到一条新的基因序列，GenBank登录号为BAD05929，经Blastn及氨基酸序列同源性比对后,发现该基因是一个未知功能序列，并命名为BmUFP。该基因全长774bp，由516bp的开放阅读框序列、67bp的 5′端非翻译区序列和191bp的3′端非翻译区序列组成，ORF共编码171个氨基酸，无内含子。编码的BmUFP蛋白预测分子量为18.49kDa，等电点为8.72, 是一个碱性蛋白，且含有一个Reeler Superfamily保守结构域。将BmUFP基因片段克隆到原核表达载体pET28a中，构建重组载体，并转化大肠杆菌 Rosetta (DE3)，经IPTG诱导后，成功表达了BmUFP蛋白，为进一步研究此基因的功能奠定了基础。
novel gene sequence was obtained from silkworm pupa cDNA library constructed by our laboratory, and the GenBank accession number was BAD05929. After Blastn based on NCBI database and alignment of deduced amino acid sequence homology, it was found to be an unknown gene, and named as BmUFP. The full length of the gene is 774bp. It contained an ORF of 516 bp，67bp nucleotide sequence in 5′UTR (untranslated region) and 191bp nucleotide sequence in 3′UTR (untranslated region) , and no introns . The BmUFP gene encoded 171amino acids, with their predicted mass 18.49kDa and isoelectric point 8.72, a basic protein. It was predicted BmUFP contained a putative conserved domain of Reeler Superfamily. The ORF fragment of BmUFP gene and pET28a expression vector were ligated. The soluble BmUFP protein was expressed induced by IPTG in E. coli Rosetta (DE3), which may lay a foundation for the study of BmUFP gene function.
聂作明、吕正兵、王丹、张耀洲、陈健、陈剑清、胡波
分子生物学昆虫学生物工程学
家蚕新基因生物信息学原核表达
silkwormnovel genebioinformaticsprokaryotic expression
聂作明,吕正兵,王丹,张耀洲,陈健,陈剑清,胡波.一个新的家蚕BmUFP基因的序列分析和原核表达[EB/OL].(2009-02-24)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/200902-1310.点此复制
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