西达本胺对luminal A型乳腺癌细胞株MCF-7增殖抑制和诱导凋亡的机制研究

Objective: To investigate effect of histone deacetylase inhibitor Chidamide on proliferation and apoptosis of luminal A breast cancer MCF-7 cells in vitro. Methods: Human breast cancer MCF-7 cell line was treated with different concentration of Chidamide (2μmol/L、 5μmol/L、10μmol/L、20μmol/L、50μmol/L、100μmol/L), and blank group was established as control. Cell growth was assessed by MTS. Morphological changes of MCF-7 were observed under inverse microscopy, and apoptosis, cell cycles as well as stem cell proportion were analyzed by flow cytometry. Western blot was used to detect expression of p53 protein between experimental and control groups. Results: Chidamide was able to inhibit proliferation of MCF-7 in vitro in positive dosage dependent manner (P＜0.05). After being treated with chidamide, cell morphology appeared great changes. Flow cytometry assay showed obvious apoptosis compared with control group with the increase of drug concentration and treatment time (P＜0.05), but no alternation of cell cycles and stem cell proportion were detected. The expression of p53 protein increased obviously after treatment compared with control group. Conclusion: Chidamide could significantly inhibit proliferation of MCF-7, and induce its apoptosis via upregulation expression of p53 protein.