HPLC测定马钱子配伍生地黄前后马钱子碱和士的宁的含量

Objective: To study the impact on Strychnine and Brucine of different proportions, which on the combination of Strychnos Nux-vomical and Radix Rehmanniae (hereinafter referred to as SR). Method: Using HPLC to determinate the mass fraction of strychnine and brucine, which in Strychnos Nux-vomical uncombined and combined with Radix Rehmanniae. Results: Compares with Strychnos single decoction, SR 1:2 decoction′s brucine and strychnine has decreased 22.46％ and 1.59％; SR 1:4 decoction′s brucine and strychnine has decreased 60.87％ and 31.36％; SR 1:6 decoction′s brucine and strychnine has increased 4.71％ and 30.91％. Conclusion: SR 1:2,1:4 decoction can reduce the toxic components of Strychnos Nux-vomical. The better proportion which can reduce the toxic components is SR 1:4.