斑马鱼发育早期CRH、UI和UII的表达及其对急性环境盐度、温度变化的响应

orticotropin releasing hormone (CRH), urotensin I (UI) and urotensin II (UII) are found throughout vertebrate species from fish to human. To further understand the role of CRH, UI and UII in teleosts during development, we investigated the expression pattern of CRH, UI, UIIα and UIIβ, and the response to acute salinity and temperature challenge during the early development of zebra?sh, Danio rerio. The results reveal that CRH, UI, UIIα and UIIβ mRNA are detected from 0hpf, and the relative expression levels alter along with development from 0hpf to 6dpf and reach the highest level at 6dpf except UIIα at 5dpf. After zebrafish at 2, 3, 4 and 5 dpf were exposed to an acute stress for 15min, no significant difference in expression levels of CRH, UI, UIIα and UIIβ mRNA was observed in response to acute osmotic (30ppt) stress compared with control, while cold stress (18℃) and hot stress (38℃) generally inhibited the mRNA levels of CRH, UI, UIIα and UIIβ at each time point apart from at 3dpf. These indicate that the contribution of CRH, UI, UIIα and UIIβ to stress response in zebrafish may be stressor-specific during early development. Overall, results from this study offer new insights into the developmental and temperature-specific function of CRH, UI, UIIα and UIIβ in zebrafish embryos and larvae.