负载叶黄素的酪蛋白-葡聚糖共聚物胶束体外消化特性研究

he controlled release of lipophilic nutrients in the human gastrointestinal fluid is a crucial factor influencing their absorption. In this study, casein-dextran conjugates through Maillard reaction were used as the matrix, and lutein was encapsulated in the conjugate micelles by self-assembly technique. The pH and ionic strength sensitivity were investigated by dynamic light scattering, and the digestion behavior was evaluated through in vitro simulated digestion experiments. It was found that the pH and ionic strength stability of conjugate micelles was significantly superior to casein micelles, and enhanced with the increase of the molecular weight of dextran moieties attached to the casein molecules. The cumulative release rate showed that lutein could be bound with protein in conjugates and uniformly dispersed in simulated gastric fluid. Whereas micelles rapidly disintegrated and released the embedding lutein within 0.5 h in simulated intestinal fluid, which led to the existence of lutein in the mixed micelles and the increase of the bioaccessibility. Therefore, casein-dextran conjugates could be used as the carrier of lutein, and effectively control its release behavior in the simulated gastrointestinal fluid.