单细胞凝胶电泳(SCGE)检测锰损伤神经元DNA
etection for DNA damage of neurons with single cells gel electrophoresis (SCGE)
目的 建立体外染锰细胞模型，探讨锰神经毒性的作用机制。方法：以原代培养的成熟皮层神经元为靶，据本室前期试验结果确定低中高不同浓度的锰液（分别为0.2mmol/L,0.6mmol/L,1.0mmol/L），与神经细胞共孵育。显微镜观察各组神经细胞形态学的变化，用单细胞凝胶电泳试验（SCGE）检测神经细胞的DNA损伤，以彗星细胞尾长及彗星样细胞百分率为评价损伤的指标。结果：光镜下可见不同浓度锰孵育后神经细胞形态学发生改变，单细胞凝胶电泳试验显示神经细胞DNA出现不同程度的损伤，彗星尾长及彗星样细胞百分率较对照组明显增加（P＜0.01）。尤以高浓度锰组损伤组严重，显著高于中低浓度组（P＜0.01）。结论：锰不但能引起体外培养的神经细胞外在的形态学损伤，还可导致神经细胞DNA的损伤。
Objective To investigate the mechanisms of Mn neurotoxicity basing on neurons model dealed with Mn in vitro.Methods: Mn of different dosages were incubated with mature cortical neurons up to optimal growth condition, Mn was designed as lower, medium and higher according our pre-experiment (that was 0.2mmol/L,0.6mmol/L,1.0mmol/L respectively）. The morphological changes in neurons were observed with light microscope and DNA damage was determined through single cells gel electrophoresis (SCGE). The length of comet tail and the percent of comet like neurons were applied to score DNA damage. Results: Mn could cause obvious morphological change under light microscope and DNA damage by SCGE.The length of comet tail and percent of comet like neurons in Mn-added groups were higher than that in control（P＜0.01）.DNA damage was specially serious in higher Mn group than that in lower and medium Mn groups（P＜0.01）.Conclusions:Mn not only could induce morphological change but also damage DNA on neurons.
鲁力、邝晓聪、陆彩玲、郭松超、陈维平
基础医学神经病学、精神病学细胞生物学
锰，单细胞凝胶电泳 (SCGE) DNA损伤
Manganese single cells gel electrophoresis (SCGE) DNA damage
鲁力,邝晓聪,陆彩玲,郭松超,陈维平.单细胞凝胶电泳(SCGE)检测锰损伤神经元DNA[EB/OL].(2007-05-11)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/200705-142.点此复制
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