合欢皮皂苷对小鼠毒性机制的研究

Objective To investigate the mechanism of total julibroside which caused the toxicity of kidney of mice. Methods After intragastric toxic doses of total julibroside for 14 days, the change of kidney was observed. The morphosis was observed by Hematoxylin Eosin staining. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) was measured by biochemitrical method. The apoptosis was detected by terminal deoxynucleotidyl transferase mediated dUTP-biotin nick-end labeling(TUNEL) method. The expression of COX-1 and COX-2 was detected by immu-histochemistry method. Results Toxic dose of total julibroside caused pathological changes in kidney, decreased the activity of SOD and GSH-PX, increased the number of apoptosis, reduced the expression of Bcl-2, increased the expression of COX-2 in epithelial cellsof Kidney Glomerulus and of COX-1 in kidney tubules. Conclusion It shows that total julibroside has toxicity to kidney. It perhaps related to increased apoptosis, decreased ability of anti-oxygen, reduced the expression of Bcl-2, and increased the expression of COX.