焦核龙眼种子败育的cDNA-AFLP分析

cDNA-AFLP technology was applied to identify diffierential genes expression between longan normal and abortive seeds. Transcript-derived fragments(TDFs) identied as dierentially expressed genes were eluted, cloned, sequenced and compared with database using BLAST at NCBI. The results showed that among 11 sequenced fragments, three TDFs presented similar with Ca2+-ATPase, cellulase, plastidic ATP/ADP transporter, respectively, whose up-regulated or down-regulated expression might influence several important metabolic pathways in the developing longan seeds and led to seed abortion; four TDFs were similar with hypothetical protein; other four TDFs were found no match to known protein and no significant similar with known nucleotide due to high E-value.