F3抑制成肌细胞C2C12的增殖与分化

Objective: To investigate the function of ATF3 in skeletal muscle regenerative myogenesis and its mechanism in C2C12 cells. Methods: Wild-type mice were employed to explore the expression of ATF3 in mouse skeletal muscle regeneration by constructing a model of ischemia-reperfusion injury in mouse skeletal muscle. A C2C12 cell model overexpressing ATF3 was constructed to study the effect of ATF3 on the proliferation and differentiation functions of myoblast C2C12 and to decipher the molecular mechanism of ATF3 function in C2C12 cells by chromatin immunoprecipitation and dual luciferase assay. Results: The expression of ATF3 decreased in skeletal muscle regeneration and C2C12 cell differentiation; Overexpression of ATF3 inhibited the proliferation and differentiation ability of C2C12 cells; ATF3 decreased the expression of PAX7 and Myh3 by targeting their promoters, and finally inhibited the proliferation and myogenic differentiation of C2C12 cells. Conclusion: ATF3 inhibits the proliferation and differentiation of myoblast C2C12 and plays a negative regulatory role in the regeneration of skeletal muscle.