In Vitro Study of Dentin Hypersensitivity Treated by Whitlockite Glass-Ceramics

Abstract The aim of this study is to evaluate the potential of a bioactive glass based on the 3CaO.P2O5- SiO2-MgO-system and its glass-ceramics containing whitlockite on the remineralization of dentin as a possible treatment to dentin hypersensitivity. For that, 40 third molar human teeth were artificially demineralized and randomly distributed in 4 groups (n = 10): G1 - Negative Control (no treatment), G2 – Positive Control (treated by Bioglass? 45S5), G3 – BG (treated by bioactive glass based on 3CaO.P2O5-SiO2-MgO-system), and G4 – BGC (treated by bioactive whitlockite glass-ceramics). After treatment, the samples were emerged in artificial saliva and stored for 7 days in a controlled temperature of 37°C. After that, ATR-FTIR was used to identify and quantitatively analyze the mineral variation of the dentin surface. The morphological aspects were evaluated by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The analysis confirmed the formation of hydroxyapatite on the surface of all the biomaterials studied, and it also showed that the results obtained with the glasss-ceramic are similar to those obtained with Bioglass?45S5 AFM and SEM examination showed that in the dentine specimens treated by bioactive glass and whitlockite glass-ceramic most of the dentinal tubules were completely occluded.