鸭茅杂种后代分子标记鉴定及遗传分析
Identification and genetic variation analysis of orchardgrass hybrids （Dactylis glomerata L.）
以140个鸭茅杂交种单株及其亲本为材料，采用SSR分子标记技术快速鉴定真假杂种。研究结果表明：SSR标记在供试材料中的扩增带型表现为互补型、父本型及其它型。利用3对特异引物A01G20、A03N16、A03K22可快速准确地鉴定出杂交种111个。试验所用的100对SSR引物中有13对引物分别在杂种和双亲之间存在扩增多态性。13对引物在供试材料中共扩增出多态性条带76条， 平均每对引物扩增出6条，多态性百分率为84.24%。供试材料具有丰富的遗传变异，利用SSR进行鸭茅杂种鉴定及遗传变异分析是可行的。
he 140 hybrids of orchardgrass （Dactylis glomerata L.）and their parental lines were tested by simple sequence repeats (SSR) markers. The amplification bands of hybrids showed complementary pattern of both parents, male pattern and other pattern. 111 hybrids can be effectively and more reliably detected by three complementary pattern primers : A01G20、A03N16、A03K22. A total of 76 polymorphic bands were detected by 13 primer pairs, with an average of 6 bands, the Percentage of polymorphic bands was 84.24%.These results suggested that there was rich genetic variation among all orchardgrass hybrids tested and SSR technique has wide prospective in hybrid identification and genetic analysis of orchardgrass.
谢文刚、蒋林峰、黄琳凯、张新全
农作物遗传学分子生物学
草原学鸭茅SSR标记杂种鉴定遗传变异分析
Grassland scienceOrchardgrass (Dactylis glomerata L.)SSR markershybrid identificationgenetic variation analysis
谢文刚,蒋林峰,黄琳凯,张新全.鸭茅杂种后代分子标记鉴定及遗传分析[EB/OL].(2013-12-19)[2025-08-24].http://www.paper.edu.cn/releasepaper/content/201312-511.点此复制
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