猪α（1, 2）岩藻糖转移酶（FUT1）基因启动子区克隆及多态性分析

he aim of the study is to provide a theoretical foundation for screening and determining stable genetic marker, as well as conducting the research of regulatory mechanism of porcine FUT1 gene.FUT1 gene upstream sequence was obtained by genome walking and analyzed by bioinformatics, and the polymorphism in wild boar and 11 different pig breeds were investigated by PCR-SSCP. The results showed that 1150 bp FUT1 gene promoter sequence successfully attained by twice genome walking and there were 3 CpG islands , the analysis of characteristics of the promoter and transcription factor binding sites showed there are Sp1, c-ETS of GATA-1 and GATA-2 existing; the results of PCR-SSCP analysis showed that there existed only a T(-726)C point mutation and 3 genotypes which was defined AA, AB and BB. Difference of genotype distribution between domestic pigs and foreign was significant.. This study further confirmed that the genetic basis which resistance to F18 E. coli was significant differences between Chinese native pig breeds and western pig breeds, which may be related to the T(-726)C point mutation in FUT1 gene promoter.