乳酸铝对原代脉络丛上皮细胞LRP-1及其配体表达的影响

Objective: In this study, we aim to investigate the effect of aluminum lactate on the expression of LRP-1 and its ligands in primary cultured choroid plexus epithelial cells (CPEC). Methods: We cultured CPECs from choroid plexus of SD rats aged 14 days. Different concentrations of aluminum lactate as control, low-, mid- and high-doses were added into the CPECs 7 days. The protein’s expression of LRP-1 were analysed by Western Blot. The genes’ expression of LRP-1, α2m, Apoj, TTR were quantified by real time PCR, Results: The proteins’expression of LRP-1 in the mid-dose, high-dose was significantly lower (P<0.05) than that in the control. The genes’expression of LRP-1, α2m, TTR, Apoj were significantly lower (P<0.05) than those in the control. Conclusion: The mechanism that aluminum lactate could induce accumulation of Aβin the brain maybe relate to the reduction in the expression of LRP-1, and its ligands of α2m, Apoj, TTR.