纳滤法分离纯化麦芽糖基-β-环糊精的研究

he product of branched cyclodextrin produced by the enzymatic method is complex and contains a large amount of maltose, which makes the process of separating and purifying the target product cumbersome and time-consuming. Membrane separation has the advantages of easy operation, no phase change, no chemical reagent addition, high separation efficiency, wide application range, and continuity. Solution of maltose and β-cyclodextrin were used as the models the branched cyclodextrin enzyme synthesis system, and the maltose and the β-CD were separated using a spiral-wound membrane with a molecular weight cut-off of 1000. The effect of operating pressure, temperature and material concentration on membrane flux, rejection rate, fractionation purity, fractionation efficiency and solute flux were discussed. Under the experimental condition, the best separation effect were appeared at 0.4 MPa, 40 C, and the material concentration was 10%. After 5 daifiltration cycles, the maltose concentration was reduced from 74.95% to 26.9%, and the branched cyclodextrin concentration was increased from 9.5% to 31.05%. It can be seen that nanofiltration was feasible to separate maltosyl-β-cyclodextrin, which is advantage to get high-purity branched cyclodextrin.